hek bluetm detection medium (InvivoGen)

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InvivoGen hek bluetm detection medium
Hek Bluetm Detection Medium, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 601 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek+bluetm+detection+medium/HEK-Blue+Detection/pm41916320-681-249-255
Average 96 stars, based on 601 article reviews

hek bluetm detection medium - by Bioz Stars, 2026-09

96/100 stars

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Concentration Assay:

Article Title: A single oral dose of an iso-alpha acids rich hop extract dampens the lipoteichoic acid mediated immune response of monocytes in healthy individuals.
Article Snippet: In contrast, when monocytes isolated 1 h after the Aidenbach, Germany) containing 10% fetal bovine serum (Pan-Biotech GmbH, Aidenbach, Germany) and 100 μg/ml streptomycin and 100 U/ml penicillin (Pan-Biotech GmbH, Aidenbach, Germany). .. After reaching 80% confluence, cells were challenged with 0–10 μg/ml LTA in the presence of 0–40 μg/ml iso-alpha acids dissolved in HEK-BlueTM Detection medium (InvivoGen, Toulouse, France) for 12 h. Colour changes of the medium being indicative of ligand concentration and binding were determined at 655 nm. .. Cell culture experiments with J774A.1 cells J774A.1 cells (DMSZ, Braunschweig, Germany) showing a morphology similar to macrophages/monocytes, were cultured in Dulbecco ́s Modified Eagle Medium (Pan-Biotech GmbH, Aidenbach, Germany) supplemented with 10% fetal bovine serum (Pan-Biotech GmbH, Aidenbach Germany), 100 μg/ml streptomycin and 100 U/ml penicillin (Pan-Biotech GmbH, Aidenbach, Germany) at 37 °C in a humidified 5% CO2 atmosphere.

Article Title: A single oral dose of an iso-alpha acids rich hop extract dampens the lipoteichoic acid mediated immune response of monocytes in healthy individuals
Article Snippet: In brief, cells were grown in a humidified, 5% carbon dioxide atmosphere using DMEM media (Pan-Biotech GmbH, Aidenbach, Germany) containing 10% fetal bovine serum (Pan-Biotech GmbH, Aidenbach, Germany) and 100 μg/ml streptomycin and 100 U/ml penicillin (Pan-Biotech GmbH, Aidenbach, Germany). .. After reaching 80% confluence, cells were challenged with 0–10 μg/ml LTA in the presence of 0–40 μg/ml iso-alpha acids dissolved in HEK-BlueTM Detection medium (InvivoGen, Toulouse, France) for 12 h. Colour changes of the medium being indicative of ligand concentration and binding were determined at 655 nm. .. J774A.1 cells (DMSZ, Braunschweig, Germany) showing a morphology similar to macrophages/monocytes, were cultured in Dulbecco ́s Modified Eagle Medium (Pan-Biotech GmbH, Aidenbach, Germany) supplemented with 10% fetal bovine serum (Pan-Biotech GmbH, Aidenbach Germany), 100 μg/ml streptomycin and 100 U/ml penicillin (Pan-Biotech GmbH, Aidenbach, Germany) at 37 °C in a humidified 5% CO2 atmosphere.

Binding Assay:

Recombinant:

Article Title: Development of Recombinant Anti-TLR2 Antibodies and PLGA Nanoparticle-based Gene Therapy for the Treatment of Neuropathic Pain
Article Snippet: HEK-BlueTM hTLR2 cells (InvivoGen, San Diego, CA, USA) were seeded into 96-well plates at a density of 1 × 10 cells per well and incubated overnight at 37 °C in a humidified incubator with 5% CO 2 . .. The following day, the culture medium was replaced with 160 μL of HEK-BlueTM Detection medium (InvivoGen), and cells were treated with 20 μL of recombinant antibody solutions in PBS. .. After 1 h of antibody pretreatment, cells were stimulated with 0.5 ng/mL Pam3CSK4 (Tocris Bioscience, Bristol, UK) and incubated for 48 h. SEAP activity in the supernatant was assessed by measuring OD at 655 nm using a microplate reader (Molecular Devices, San Jose, CA, USA).

other:

Article Title: High-fat diet causes rapid loss of intestinal group 3 innate lymphoid cells through microbiota-driven inflammation and mitochondrial stress.
Article Snippet: Article High-fat diet causes rapid loss of intestinal group 3 innate lymphoid cells through microbiota-driven inflammation and mitochondrial stress

Reporter Assay:

Article Title: Trichinella spiralis -derived extracellular vesicles induce regulatory T cells and reduce airway allergy in mice
Article Snippet: .. For the reporter assay, 2 × 104 cells were seeded in a 96-well plate in 180 μL of HEK-BlueTM Detection Medium (Invivogen). .. Two-fold serial dilutions of the TLR4 ligand (ultrapure LPS from E. coli O111:B4, Invivogen) were prepared in a range of 500 ng/mL to 0.24 ng/mL, along with TsEVs at 1.56×107 and 5.2×107, in 20 μL of HEKBlueTM Detection Medium (corresponding to 0.78×109 and 2.6×109 Particles/mL), and added to the cells.

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